Intro to Biotechnology
Pre-requisites – Biology I and Chemistry (may be taken at same time) 2020-2021
Location - Millersburg Area School District
Lecture and Laboratory: Monday through Friday 45 minute periods
Name and Contact Information
Phone: 717-692-2108 Ext.1139
Text: Biotechnology: A Laboratory Skills Course Second Edition (2018)
Course Description and Learning Objectives
This course is designed for juniors and seniors with at least two years of prior science including Biology. The course centers on contemporary topics in biology and biotechnology including microbiology, pathology, forensics and criminalistics, and genetic technologies. The focus will be on examination and understanding of various topics in the Biotechnological world around them and the way the quality of life can be improved through modern biological techniques. With all the popularity and relation to biotechnology, forensic sciences and criminalistics will be another aspect of study. This course integrates concepts and hands-on laboratory activities together with real-world applications to get a better understanding of the world of biotechnology and genetic engineering. Laboratory skills include DNA extraction, pipetting, calculating dilutions, making solutions, media preparation, culturing bacteria, streaking plates, restriction enzyme digestion, horizontal agarose gel electrophoresis, DNA fingerprinting, plasmid mapping, and eukaryotic cell staining. Students will learn the basics of DNA structure along with manipulation techniques and tools, including restriction enzymes, ligases, advanced cloning techniques, and CRISPR technology. They will perform PCR to identify suspect DNA, detect genetically modified material in foods, determine human relatedness, and barcode fish species. Students will learn about protein translation, protein production, and the role of proteins in drug discovery. Finally, the students will also examine the ethical implications of these topics and technologies, studying their impact on their lives as well as society’s in general.
Prerequisites and Co-requisites
Students must successfully complete Biology I and have either passed Chemistry or be taking it concurrently.
Students enrolling in this course are expected to have the following skills: college-level reading comprehension; college-level composition; application of intermediate algebra; and proficiency with computer programs such as Google slides and Google docs
Textbooks and other course materials
Text: Biotechnology: A Laboratory Skills Course Second Edition (2018)
Lab Notebook: A lab notebook will be provided
Tests/Quizzes about 50%
Laboratory Reports/Essays about 40%
Homework/Classwork about 10%
- ASSESSMENT: A variety of assessments will be used in this course, but the majority of your grade will be determined by quizzes and tests. A unit test will be given upon completion of each unit of study. Questions on quizzes and tests will include material covered during class, in assigned readings and from laboratories. Projects may also be given for various units of study. Additionally, since labs are a major part of the Biotechnology course, laboratory reports will be graded.
**Since this course will be offered for college credit through Harrisburg University, two Key Assignments will be required throughout the year. Requirements for both assignments will be determined by Harrisburg University. One is an applications essay, and one is a poster-based project. Student work will be scanned and submitted to the college. Both assignments must be completed, and the grades will factor into the final grade for this course.
- EVALUATION: Evaluation criteria include participating in and completing classroom activities, working cooperatively in group assignments, correctly answering questions on quizzes/tests, completion of assigned projects, communication of laboratory data in a correct and complete manner, and demonstrating proper lab techniques and safety.
- LATE WORK: YOU ARE RESPONSIBLE FOR TURNING IN ALL ASSIGNMENTS ON TIME. Points will be taken off for late work.
- MAKE UP WORK: According to district policy, students must have the ability to make up any missed work for verified absences. It is your responsibility to make arrangements to get the work that you miss when you are absent. If you miss a quiz or test, it is your responsibility to schedule a time to take it. Labs are particularly difficult to make up so, make every effort to attend all classes where we will be conducting a lab.
- Plagiarism Statement: Plagiarism, in any extent, will not be tolerated under any circumstance. Incidences of plagiarism will result in a grade of F for that assignment, and a formal complaint will be filed with the Dean of Student Affairs/Provost. Students will have an opportunity to appeal the complaint. Any students uncertain about the definition of plagiarism are encouraged to talk with their instructor, an advisor in the student success center, or the campus librarian.
- Students will complete a lab report for each laboratory activity. Lab reports will be graded according to the following table:
Lab Report Grade Sheet:
Purpose / Introduction (5 pts.)
Methods (5 pts.)
Equations / Calculations (if applicable) (5 pts.)
Data Tables / Figures/photos as applicable/results (5 pts.)
Conclusions (10 pts.)
Writing / Grammar (5 pts.)
Spelling (5 pts.)
Report Total (40 points)
Chapter 1 – Biotechnology Industry
1.1 – What is Biotechnology?
1.2 – Who uses Biotechnology?
1.3 – The Biotechnology Industry
1.4 – Governmental Regulation of Biotechnology
1.5 – Industry Practices
1.6 – Biotechnology Company Structure
1.7 – Careers in Biotechnology
Chapter 2 – Laboratory Skills
2.1 – Laboratory Safety
2.2 – Laboratory Notebooks
2.3 – Laboratory Equipment
2.4 – Numerical Data
2.5 – Preparing Solutions
Chapter 3 – Microbiology and Cell Culture
3.1 – Microbiology and Cell Biology
3.2 – Bacteria
3.3 – Uses of Bacteria in Biotechnology
3.4 – Culturing Bacteria in the Laboratory
3.5 – Microbiological Techniques
3.6 – Eukaryotic Cells
3.7 – Uses of Eukaryotic Cells in Biotechnology
3.8 – Eukaryotic Cell Culture
Chapter 4 – DNA Structure and Analysis
4.1 – Molecular Biology
4.2 – DNA Structure
4.3 – Recombinant DNA Technology
4.4 – DNA Analysis Techniques
4.5 – Restriction Enzymes as Forensic Tools
Chapter 5 – Bacterial Transformation and Plasmid Purification
5.1 – History of Bacterial Transformation and Plasmids Bacteria
5.2 – Transforming Cells
5.3 – Plasmid Purification and Quantitation
Chapter 6 – The Polymerase Chain Reaction
6.1 – Invention of PCR
6.2 – What is PCR?
6.3 – Thermal Cyclers
6.4 – Types of PCR
6.5 – PCR Optimization
6.6 – Techniques Based on PCR
6.7 – Real-World Applications of PCR
Chapter 7 – Protein Structure and Analysis
7.1 – Protein Synthesis
7.2 – Protein Structure
7.3 – Proteins in Biology
7.4 – Proteins in Biotechnology
7.5 – Methods of Protein Analysis
7.6 – Protein Production in Industry
Chapter 8 – Immunological Applications
8.1 – The Immune System
8.2 – Antibodies as Tools
8.3 – Immunoassays
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